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immunofluorescence if staining analysis  (Proteintech)


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    Proteintech immunofluorescence if staining analysis
    Overexpression ACSL4 increases the therapeutic efficacy of anti-PD-1 antibody in vivo. (A) A schematic view of the treatment plan, (B) Images of isolated tumors from xenograft mice that received indicated treatment. Tumor volumes and weights in each group were calculated and displayed on the right. (C) <t>Immunofluorescence</t> staining of ferroptosis marker PTGS2 in isolated xenograft tumors with indicated treatment. Scale bar = 100 μm. Data represent the mean ± SEM of triplicates. P value was calculated by two-tailed Student’s t-test. ** P < 0.01, *** P < 0.001. n = 6
    Immunofluorescence If Staining Analysis, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunofluorescence if staining analysis/product/Proteintech
    Average 96 stars, based on 237 article reviews
    immunofluorescence if staining analysis - by Bioz Stars, 2026-06
    96/100 stars

    Images

    1) Product Images from "Lipid metabolism-related genes correlate with immune microenvironment and regulate the efficacy of immunotherapy via ferroptosis in melanoma"

    Article Title: Lipid metabolism-related genes correlate with immune microenvironment and regulate the efficacy of immunotherapy via ferroptosis in melanoma

    Journal: Discover Oncology

    doi: 10.1007/s12672-025-04163-x

    Overexpression ACSL4 increases the therapeutic efficacy of anti-PD-1 antibody in vivo. (A) A schematic view of the treatment plan, (B) Images of isolated tumors from xenograft mice that received indicated treatment. Tumor volumes and weights in each group were calculated and displayed on the right. (C) Immunofluorescence staining of ferroptosis marker PTGS2 in isolated xenograft tumors with indicated treatment. Scale bar = 100 μm. Data represent the mean ± SEM of triplicates. P value was calculated by two-tailed Student’s t-test. ** P < 0.01, *** P < 0.001. n = 6
    Figure Legend Snippet: Overexpression ACSL4 increases the therapeutic efficacy of anti-PD-1 antibody in vivo. (A) A schematic view of the treatment plan, (B) Images of isolated tumors from xenograft mice that received indicated treatment. Tumor volumes and weights in each group were calculated and displayed on the right. (C) Immunofluorescence staining of ferroptosis marker PTGS2 in isolated xenograft tumors with indicated treatment. Scale bar = 100 μm. Data represent the mean ± SEM of triplicates. P value was calculated by two-tailed Student’s t-test. ** P < 0.01, *** P < 0.001. n = 6

    Techniques Used: Over Expression, Drug discovery, In Vivo, Isolation, Immunofluorescence, Staining, Marker, Two Tailed Test



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    Image Search Results


    Overexpression ACSL4 increases the therapeutic efficacy of anti-PD-1 antibody in vivo. (A) A schematic view of the treatment plan, (B) Images of isolated tumors from xenograft mice that received indicated treatment. Tumor volumes and weights in each group were calculated and displayed on the right. (C) Immunofluorescence staining of ferroptosis marker PTGS2 in isolated xenograft tumors with indicated treatment. Scale bar = 100 μm. Data represent the mean ± SEM of triplicates. P value was calculated by two-tailed Student’s t-test. ** P < 0.01, *** P < 0.001. n = 6

    Journal: Discover Oncology

    Article Title: Lipid metabolism-related genes correlate with immune microenvironment and regulate the efficacy of immunotherapy via ferroptosis in melanoma

    doi: 10.1007/s12672-025-04163-x

    Figure Lengend Snippet: Overexpression ACSL4 increases the therapeutic efficacy of anti-PD-1 antibody in vivo. (A) A schematic view of the treatment plan, (B) Images of isolated tumors from xenograft mice that received indicated treatment. Tumor volumes and weights in each group were calculated and displayed on the right. (C) Immunofluorescence staining of ferroptosis marker PTGS2 in isolated xenograft tumors with indicated treatment. Scale bar = 100 μm. Data represent the mean ± SEM of triplicates. P value was calculated by two-tailed Student’s t-test. ** P < 0.01, *** P < 0.001. n = 6

    Article Snippet: The primary antibodies and dilutions for western blotting (WB) and immunofluorescence (IF) staining analysis are listed below: ACSL4 (22401-1-AP, Proteintech, Wuhan, China; 1:100 for IHC, 1:100 for IF and 1:5 000 for WB), ALOX5 (10021-1-Ig, Proteintech; 1:400 for IHC, and 1:500 for WB), PTGS2 (66351-1-Ig, Proteintech; 1:400 for IF), CD8α(ab22378,, Abcam; 1:300 for IF), ACTIN (66009-1-Ig, Proteintech; 1:10 000 for WB).

    Techniques: Over Expression, Drug discovery, In Vivo, Isolation, Immunofluorescence, Staining, Marker, Two Tailed Test

    High elevated NUSAP1 expression in LUAD. (A) The significant upregulation of NUSAP1 in tumor tissues. (B) The remarkable capacity of NUSAP1 to distinguish between tumor and normal samples. (C) NUSAP1 expression in the UALCAN database. (D) The promoter methylation levels of NUSAP1. (E) NUSAP1 expression between tumor and normal samples in the Human Protein Atlas database. (F) The result of qRT-PCR. (G) The result of IHC assays. (H) The result of immunofluorescence staining analysis. (I) The relationship between NUSAP1 expression and clinicopathological features. ns, not significant; *p < 0.05; **p <0.01; ***p < 0.001.

    Journal: International Journal of Medical Sciences

    Article Title: Comprehensive multi-omics analysis identifies NUSAP1 as a potential prognostic and immunotherapeutic marker for lung adenocarcinoma

    doi: 10.7150/ijms.102331

    Figure Lengend Snippet: High elevated NUSAP1 expression in LUAD. (A) The significant upregulation of NUSAP1 in tumor tissues. (B) The remarkable capacity of NUSAP1 to distinguish between tumor and normal samples. (C) NUSAP1 expression in the UALCAN database. (D) The promoter methylation levels of NUSAP1. (E) NUSAP1 expression between tumor and normal samples in the Human Protein Atlas database. (F) The result of qRT-PCR. (G) The result of IHC assays. (H) The result of immunofluorescence staining analysis. (I) The relationship between NUSAP1 expression and clinicopathological features. ns, not significant; *p < 0.05; **p <0.01; ***p < 0.001.

    Article Snippet: Immunofluorescence staining analysis from the Human Protein Atlas database highlighted the predominant localization of NUSAP1 within the nucleus (Figure H).

    Techniques: Expressing, Methylation, Quantitative RT-PCR, Immunofluorescence, Staining